Variant galactokinase kinetic data
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Resource or Project Abstract
It is proposed to assess the potential of two related recombinant human enzymes ? galactokinase and N-acetylgalactosamine kinase ? as catalysts for low environmental impact site- and stereospecific phosphorylation of sugars.
This will be achieved by:
1. Rational, structure-based mutagenesis of both enzymes to alter the substrate specificity thus providing new synthetic routes to sugar and N-acetyl sugar phosphates.
2. Determining the effects of temperature, organic solvents and covalent immobilisation to a solid substrate (such as agarose or sepharose) on the enzymes to assess their suitability for use in scaled-up industrial processes.
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Data, Files, Information Objects Related To This Project Resource
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| Attachment Name and Download Link |
|---|
| Att 1 GALK1.zip (18.04 Mb) |
Suggested Citation Information
The information supplied in the table below should be provided with all citations to this electronic resource. You are requested to format each of the fields below as required by the specific bibliographical style you are using.
| Author(s) | Timson, D. |
| Title Of Website | Secure Archive For Environmental Research Data |
| Publication Information | Variant galactokinase kinetic data |
| Name of Organisation | Environmental Protection Agency Ireland |
| Electronic Address or URL | https://eparesearch.epa.ie/safer/resource?id=76dec008-1f7b-102f-a0a4-f81fb11d7d1c |
| Unique Identifier | 76dec008-1f7b-102f-a0a4-f81fb11d7d1c |
| Date of Access | Last Updated on SAFER: 2024-09-19 |
An example of this citation in proper usage:
Timson, D. "Variant galactokinase kinetic data". Associated datasets and digitial information objects connected to this resource are available at: Secure Archive For Environmental Research Data (SAFER) managed by Environmental Protection Agency Ireland https://eparesearch.epa.ie/safer/resource?id=76dec008-1f7b-102f-a0a4-f81fb11d7d1c (Last Accessed: 2024-09-19)
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Access Information For This Resource
| SAFER-Data Display URL | https://eparesearch.epa.ie/safer/iso19115/display?isoID=213 |
| Resource Keywords | Galactokinase, GALK1, enzyme, variant, specificity change, green chemistry |
| EPA/ERTDI/STRIVE Project Code | 2007-PhD-ET-9 |
| EPA/ERTDI/STRIVE Project Theme | Environmental Technologies |
| Resource Availability: |
 Public-Open |
| Limitations on the use of this Resource | Data may be used freely; however, if input is required from the data provided this should be acknowledged (usually by co-authorship) in any publications.
Users of the data must cite this resource AND: Kristiansson H & Timson DJ (2011) Increased Promiscuity of Human Galactokinase Following Alteration of a Single Amino Acid Residue Distant from the Active Site. Chembiochem. doi: 10.1002/cbic.201100308 |
| Number of Attached Files (Publicly and Openly Available for Download): | 1 |
| Project Start Date | Monday 1st October 2007 (01-10-2007) |
| Earliest Recorded Date within any attached datasets or digital objects | Monday 1st October 2007 (01-10-2007) |
| Most Recent Recorded Date within any attached datasets or digital objects | Friday 31st December 2010 (31-12-2010) |
| Published on SAFER | Wednesday 24th August 2011 (24-08-2011) |
| Date of Last Edit | Wednesday 24th August 2011 at 10:13:40 (24-08-2011) |
| Datasets or Files Updated On | Wednesday 24th August 2011 at 09:39:24 (24-08-2011) |
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Geographical and Spatial Information Related To This Resource
Description of Geographical Characteristics of This Project or Dataset
NONE
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Supplementary Information About This Resource
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| Lineage information about this project or dataset |
| Sugar phosphates are difficult to make by traditional synthetic chemistry methods. Furthermore, these methods tend to be energy intensive, require organic solvents and produce environmentally harmful waste products. Enzymes which catalyse the site- and stereo-specific phosphorylation of sugars (eg galactokinase) are a potential way around this problem. However, enzymes are generally highly specific and need to be modified in order to increase the range of substrates they will act upon. Some work has already been carried out (by others) on bacterial galactokinases which shows single amino acid changes in the protein can result in radical changes in specificity. |
| Supplementary Information |
| Data is provided as Excel files and also GraphPad Prism files. Data is provided "as is" and no warranty is made. |
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