Variant galactokinase models

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Resource or Project Abstract

It is proposed to assess the potential of two related recombinant human enzymes ? galactokinase and N-acetylgalactosamine kinase ? as catalysts for low environmental impact site- and stereospecific phosphorylation of sugars.

This will be achieved by:
1. Rational, structure-based mutagenesis of both enzymes to alter the substrate specificity thus providing new synthetic routes to sugar and N-acetyl sugar phosphates.
2. Determining the effects of temperature, organic solvents and covalent immobilisation to a solid substrate (such as agarose or sepharose) on the enzymes to assess their suitability for use in scaled-up industrial processes.

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Contact Information for This Resource

Dr. David Timson
Queen's University Belfast

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Data, Files, Information Objects Related To This Project Resource

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Suggested Citation Information

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Author(s)Timson, D.
Title Of WebsiteSecure Archive For Environmental Research Data
Publication InformationVariant galactokinase models
Name of OrganisationEnvironmental Protection Agency Ireland
Electronic Address or URL
Unique Identifier76497ae8-1f7e-102f-a0a4-f81fb11d7d1c
Date of AccessLast Updated on SAFER: 2024-07-13

An example of this citation in proper usage:

Timson, D.   "Variant galactokinase models". Associated datasets and digitial information objects connected to this resource are available at: Secure Archive For Environmental Research Data (SAFER) managed by Environmental Protection Agency Ireland (Last Accessed: 2024-07-13)


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Access Information For This Resource

SAFER-Data Display URL
Resource KeywordsGALK1, galactokinase, protein modelling
EPA/ERTDI/STRIVE Project Code2007-PhD-ET-9
EPA/ERTDI/STRIVE Project ThemeEnvironmental Technologies
Resource Availability: Any User Can Download Files From This Resource
Limitations on the use of this ResourceFree access is permitted. If users wish to involve the data owners in further analysis, this should be acknowledged in any publications, usually by co-authorship.

Users of the data must cite this resource AND:
Kristiansson H & Timson DJ (2011) Increased Promiscuity of Human Galactokinase Following Alteration of a Single Amino Acid Residue Distant from the Active Site. Chembiochem. doi: 10.1002/cbic.201100308
Number of Attached Files (Publicly and Openly Available for Download): 3
Project Start Date Monday 1st October 2007 (01-10-2007)
Earliest Recorded Date within any attached datasets or digital objects Monday 1st October 2007 (01-10-2007)
Most Recent Recorded Date within any attached datasets or digital objects Friday 31st December 2010 (31-12-2010)
Published on SAFERWednesday 24th August 2011 (24-08-2011)
Date of Last EditWednesday 24th August 2011 at 10:12:37 (24-08-2011)
Datasets or Files Updated On Wednesday 24th August 2011 at 10:10:48 (24-08-2011)

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Description of Geographical Characteristics of This Project or Dataset

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Supplementary Information About This Resource

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Lineage information about this project or dataset
Sugar phosphates are difficult to make by traditional synthetic chemistry methods. Furthermore, these methods tend to be energy intensive, require organic solvents and produce environmentally harmful waste products. Enzymes which catalyse the site- and stereo-specific phosphorylation of sugars (eg galactokinase) are a potential way around this problem. However, enzymes are generally highly specific and need to be modified in order to increase the range of substrates they will act upon. Some work has already been carried out (by others) on bacterial galactokinases which shows single amino acid changes in the protein can result in radical changes in specificity.
Supplementary Information
Files are in Protein DataBank (pdf) format. Some other files may be included.
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